ABSTRACT.- Fusarium head blight (FHB) is a destructive disease of cereal grains caused by several Fusarium species, of which Fusarium graminearum is considered the primary causal agent. In this work 586 pure cultures of Fusarium spp. were obtained from infected grains, of which 64.9% belonged to the Fusarium graminearum species complex. 96.4% of those isolates had 15-acetyldeoxynivalenol genotype and the rest exhibited Nivalenol genotype. The second most predominant species was F. poae (19.1%) followed by F. avenaceum (8.2%) and F. tricinctum (4.6%). An increase in the tolerance to tebuconazole of Uruguayan Fusarium spp. isolates was detected.© 2022 Informa UK Limited, trading as Taylor & Francis Group