This study aimed to quantify the effect of dairy cattle urine N on soil NH4+, NO3-, pH, as well as on nitrous oxide emissions in a mixed pasture soil. The study was conducted at the dairy unit of INIA - La Estanzuela, Colonia, Uruguay (34°21'04.2"S 57°41'32.4"W). The experiment was arranged as a complete block randomized statistical design (CRBD) with 4 repetitions.