ABSTRACT.- Colloquially known as Soybean Stem Fly, Melanagromyza sojae (Zehntner, 1901) (Diptera, Agro-myzidae) is an oligophagous pest of plants in the family Fabaceae, including Uruguay?s biggest commodity crop, Soybean (Glycine max (L.) Merr.). To our knowledge, this is the first scientific record of M. sojae in Uruguay, and we confirm its identity through using DNA barcoding. Characteristic damage to host plants and immature stages of M. sojae were confirmed in Dolores and Colonia, Uruguay. © The authors.