ABSTRACT.- Genome editing success in crop species is largely dependent on the availability of highly efficient plant transformation protocols. Tomato (Solanum lycopersicum) was the first dicotyledonous crop to be successfully mutagenized using CRISPR-Cas9. Despite many efforts, no standardized, simple protocol is available for non-model tomato genotypes. With the increasing availability of gene editing tools, the transformation of elite tomato breeding lines has gained importance because mutant variants can be easily incorporated into breeding programs.

ABSTRACT.- The entomopathogenic fungus Beauveria bassiana is widely used as a biocontrol agent, but its efficacy varies depending on the target insect species. Piezodorus guildinii, a major soybean pest, exhibits low susceptibility to B. bassiana. Thus, biocontrol of this pest requires improving virulence of B. bassiana. Here, we used genomic and transcriptomic analyses to identify novel genes associated with the enhanced virulence of B. bassiana strain ILB308 when exposed to the insect epicuticular hydrocarbon n-pentadecane.

CONTENIDO: Ganadería y Emisiones de Gases de Efecto Invernadero (GEI). -- El metano a partir de la fermentación ruminal. -- Mediciones de Eficiencia y Emisiones de Metano. -- Las pruebas se llevan adelante en la central Kiyu / Hereford. -- Pruebas de Engorde Novillos Hereford (2022/2023). -- Animales eficientes vs ineficientes. --

CONTENT: Enteric Methane Emissions Measures. -- Experimental Design. -- Operational Procedure Configuration. -- Diet Composition. -- Frequency distribution of visits and diurnal pattern daily CH4emissions. -- Descriptive and Correlations to Methane Emission. -- Methane emissions and feedintake. -- High efficiency Vs Low efficiency. --

ABSTRACT.- The observation time and water background can affect the remote sensing estimates of the nitrogen (N) content in rice crops. This makes the use of vegetation indices (VIs) for N status monitoring and topdressing recommendations challenging, as the timing of panicle initiation and the water level in bays usually differ between farms even when managed using the same irrigation technique.

ABSTRACT.- Intoxication of sheep and cattle by Astylus atromaculatus recently occurred in Uruguay and Argentina in association with severe drought. Although the disease was experimentally reproduced in sheep in the 1970s, there is limited information on clinical and pathologic findings of sheep experimentally intoxicated by this beetle. Here, we described the clinical, gross, and microscopic findings in 3 sheep orally dosed with A. atromaculatus (treatment group, TG) and in 2 control sheep (control group, CG) dosed with distilled water.