ABSTRACT.- Tree plantations are expanding in southern South America and their effects on ecosystem services, particularly climate regulation, are still not well understood. Here, we used remote sensing techniques and a paired design to analyze 33,000 ha of Pinus plantations along a broad geographical and environmental gradient (26-43° South latitude, 54?72° West longitude). Radiation interception, surface temperature, evapotranspiration, and albedo were assessed both in tree plantations stands and in adjacent uncultivated areas. Additionally, the climatic impact of tree plantations was quantified by analyzing changes in atmospheric radiative forcing and its carbon (C) equivalent. Tree plantations intercepted more radiation when replacing steppes, grasslands, and shrublands but not when replacing forests. The control exerted on radiation interception by precipitation decreased in both space and time after tree plantation. Furthermore, evapotranspiration notably increased in tree plantations. The lower albedo of tree plantations compared to uncultivated adjacent areas induces global warming through the biophysical pathway. Thus, the climate benefits of afforestation through C sequestration can be counteracted by 18 to 83 % due to albedo changes. It is necessary to fully consider the biophysical effects and water footprint of tree plantations in public policies that promote them, as well as in international carbon accounting mechanisms. © 2024 Elsevier B.V.