ABSTRACT.- A simple and fast analytical method was developed and applied to assess the effect of two forms of zinc fertilization on a pecan tree cultivar in Uruguay: fertigation and foliar application with a specially formulated fertilizer. Zinc content was determined in 36 leaf samples from two crop cycles: 2020-2021 and 2021-2022. Fresh samples were dried, ground, and sieved. Analytical determinations were performed by flame atomic absorption spectrometry (FAAS, considered a standard method) and energy dispersive X-ray spectrometry (EDXRF, the proposed method). © 2025 by the authors.

ABSTRACT.- Key message: Eight QTL conferring additive APR to YR were identified in wheat germplasm using GWAS. The high accuracy of GP models supports the feasibility of accelerating breeding for YR resistance. Abstract: Wheat yellow rust (YR), caused by Puccinia striiformis f. sp. tritici (Pst), is among the most devastating diseases affecting wheat worldwide. Since 2000, YR has expanded into regions where it was previously not considered an economically important disease. The deployment of YR-resistant cultivars remains the most effective and sustainable control strategy.

ABSTRACT.- This study analyzed the impact of land use and cover changes, along with climate variability, on water consumption by quantifying actual evapotranspiration ((Formula presented.)) in the Tres Cruces River basin (TCR) in Uruguay. Using Landsat 8 and 9 images from 2014 to 2024, the SAFER method (Simple Algorithm for Evapotranspiration Retrieving), applied for the first time in Uruguay, estimated (Formula presented.) for natural vegetation (grasslands and riparian forests) and commercial afforestation areas.

In the original article [1], the omission of two critical references led to a shift in the citation numbering throughout the discussion section, which significantly impacts the interpretation of the text.

ABSTRACT.- Ovine footrot has Dichelobacter nodosus as the primary pathogen, and it is characterized by its infectious and multifactorial nature, such as environmental conditions, management practices, and host susceptibility, leading to variable prevalence and economic impacts across regions. The present study investigated factors associated with footrot scores in individual sheep from a non-probabilistic sample of 60 flocks enrolled by the Uruguayan Wool Secretariat, from which 6,139 sheep had their feet clinically evaluated from 2021 to 2024. PCR was employed to confirm D.

ABSTRACT.- Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by severe joint inflammation, synovial hyperplasia and degradation of the cartilage and bone in the joint. Patients with RA have an amplified T helper (Th) 1 and Th17 immune response and production of autoantibodies by autoreactive B cells. In the joint, macrophages mediate bone destruction and maintain the inflammatory process in RA.

While agriculture is crucial to feeding people, it is strongly impacted by climate change and variability, and also contributes to it. Agriculture and land use change are responsible for around ¼ of greenhouse gas emissions from human activities(1). Climate change already impacts the quantity, quality and geographic distribution of food production from agriculture. In this special issue, entitled "Climate change and variability impacts on agriculture", the selected articles address different issues.

ABSTRACT. - Zygote microinjection is considered the most suitable technique to introduce CRISPR/Cas9 reagents for efficient genome editing in livestock. In this study, zygote electroporation was evaluated as an alternative to microinjection for CRISPR/Cas9-mediated genome editing in sheep. Four experiments were conducted on 3548 cumulusoocyte complexes. Acid Tyrode's solution (AT) was used to partially degrade the zona pellucida (ZP) to improve reagent entry, resulting in ZP thinning with longer AT exposure (P < 0.05).

ABSTRACT.- The antifungal activity of Chenopodium ambrosioides essential oil against Penicillium digitatum was evaluated in vitro (liquid phase) and in vivo on artificially inoculated Okitsu Satsuma mandarin (Citrus unshiu). In vitro, the essential oil exhibited a fungicidal effect, with 100% effectiveness at a minimum inhibitory concentration of 29 uL/mL, and a minimum fungicidal concentration of 125 uL/mL. However, in vivo, P. digitatum grew on over 80% of treated fruit, showing no significant

ABSTRACT.- The intensification of irrigated rice production systems requires a comprehensive assessment of the environmental impact of pesticide use. Here, we investigated the behavior of the herbicide glyphosate (GLY) and its metabolite aminophosphonic acid (AMPA), throughout four rice rotations (continuous rice cropping (RC), ricesoybean (R-S), rice-short term pasture (R-PS) and rice-long term pasture R-PL) reflecting different agricultural intensification scenarios in a long-term experimental (LTE) site. © 2025 Elsevier B.V.