ABSTRACT.- Grasslands are among the most human-modified biomes worldwide due to the expansion of croplands and afforestation. In the scenario of productive intensification, it is necessary to generate alternatives to model land-cover changes and their environmental consequences. This study aimed to generate land-cover projections and quantify the impact of these changes on ecosystem service supply in Uruguay. To achieve this, we used land-cover maps, Markovchain models and an ecosystem services supply index (ESSI).

ABSTRACT.- The objective of this systematic review and meta-analysis was to evaluate the effect of probiotic supplementation on ADG, feed intake, and feed efficiency (FE) of dairy calves. A secondary objective was to assess outcomes stratified by probiotic type. Our study included quasirandomized and randomized controlled trials written in English, Spanish, or Portuguese that assessed the effects of probiotic supplementation on the growth of dairy calves. No restrictions were placed on the publication year.

ABSTRACT.- In a collaborative effort with private agents of the oilseed industry, INIA conducted a research study to determine the feasibility of framing soybean production in Uruguay into a sustainable development pathway. A spatial model based on land suitability analysis and the imposition of other soil restrictions (risk erosion, current regulations, and permanent soil uses) was adopted to estimate potential soybean yields and the most suitable cropping areas in the country.

ABSTRACT.- Pregnancy rates following embryo transfer (ET) in cattle may be influenced by transient inflammatory responses triggered during the procedure. The objective of this study was to evaluate the effect of tolfenamic acid (TA), a nonsteroidal anti-inflammatory drug (NSAID), on pregnancy rates in embryo-recipient females. A total of 1431 recipients (367 heifers and 1064 cows) were enrolled in 34 ET programs conducted on 24 commercial farms in Uruguay, using either in vivo-derived or in vitro-produced embryos, fresh or frozen.

ABSTRACT.- Societal Impact Statement: Arbuscular mycorrhizal (AM) fungi support plant development by enhancing growth and resistance to pathogens through mycorrhiza-induced resistance (MIR). However, the varying capacities of individual AM fungal species to induce MIR are not well-understood, limiting their agricultural potential. This study reveals that specific AM fungal isolates differ in their ability to enhance tomato growth and reduce biomass losses due to Rhizoctonia solani infection, a major root pathogen.

ABSTRACT.- Background: Likelihood-based inferences such as variance components estimation and hypothesis testing need logarithms of the determinant (log-determinant) of high dimensional matrices. Calculating the log-determinant is memory and time-consuming, making it impossible to perform likelihood-based inferences for large datasets. Conclusions: The method presented in this study allows to approximate the log-determinant of positive semi-definite matrices and, therefore, the likelihood for datasets of any size.

ABSTRACT.- Fusarium graminearum sensu stricto (F. graminearum s.s) and Fusarium proliferatum are species frequently isolated from sorghum grains. However, ecophysiology studies of these Fusarium species have not been carried out on sorghum grains. For this reason, the aim of this work was to evaluate the effects of different water activity levels (0.95, 0.98, 0.995) and temperatures (15 ◦C, 25 ◦C, 30 ◦C) on the mycelial growth and mycotoxin production of these species on a sorghum grain substrate.

ABSTRACT.- A simple and fast analytical method was developed and applied to assess the effect of two forms of zinc fertilization on a pecan tree cultivar in Uruguay: fertigation and foliar application with a specially formulated fertilizer. Zinc content was determined in 36 leaf samples from two crop cycles: 2020-2021 and 2021-2022. Fresh samples were dried, ground, and sieved. Analytical determinations were performed by flame atomic absorption spectrometry (FAAS, considered a standard method) and energy dispersive X-ray spectrometry (EDXRF, the proposed method). © 2025 by the authors.

ABSTRACT. - Zygote microinjection is considered the most suitable technique to introduce CRISPR/Cas9 reagents for efficient genome editing in livestock. In this study, zygote electroporation was evaluated as an alternative to microinjection for CRISPR/Cas9-mediated genome editing in sheep. Four experiments were conducted on 3548 cumulusoocyte complexes. Acid Tyrode's solution (AT) was used to partially degrade the zona pellucida (ZP) to improve reagent entry, resulting in ZP thinning with longer AT exposure (P < 0.05).

ABSTRACT.- The antifungal activity of Chenopodium ambrosioides essential oil against Penicillium digitatum was evaluated in vitro (liquid phase) and in vivo on artificially inoculated Okitsu Satsuma mandarin (Citrus unshiu). In vitro, the essential oil exhibited a fungicidal effect, with 100% effectiveness at a minimum inhibitory concentration of 29 uL/mL, and a minimum fungicidal concentration of 125 uL/mL. However, in vivo, P. digitatum grew on over 80% of treated fruit, showing no significant