ABSTRACT. As the biodiversity crisis worsens, its impact on economic and social development is becoming increasingly apparent. Strategies to tackle environmental degradation and climate change must therefore focus on ensuring that ecosystems can respond to high levels of pressure while continuing to provide services indispensable to human development and to life. Evidence is pointing to a bleak outlook if we continue on our current trajectory. A recent assessment by the Inter-governmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) showed that we are not on track to meet goals for the conservation of biodiversity or its sustainable use. According to the report, the only way to meet international targets on biodiversity (Aichi targets) and sustainable development (Agenda 2030) is through truly transformative change. A crucial step in this direction will be getting consensus on the need for a global agreement on the conservation and sustainable use of biodiversity for the post-2020 period. The European Union (EU) is committed to contributing to this outcome. Through initiatives like B4Life and the European Green Deal1, it promotes biodiversity conservation and sustainable development, in developing countries as well as on a global scale. Based on the findings of recent studies and the input of numerous local and international specialists, Larger than Jaguars provides guidelines for a strategic approach to biodiversity conservation for Latin America and the Caribbean (LAC). Similar detailed studies were conducted by the European Commission in Africa (Larger than Elephants) and in Asia (Larger than Tigers), providing a valuable basis for decisions in biodiversity conservation aligned with human development. The findings will not only feed the strategy of the EU to establish strong partnerships with LAC based on common values and shared interests, but are also meant to contribute to converging actions by all stakeholders in favour of ecosystem conservation and enhancement. This proposal is in line with the political document European Union, Latin America and the Caribbean: joining forces for a common future (16 April, 2019), presented by the EU High Representative and the European Commission. This latest document emphasises the importance of preserving biodiversity, moving towards a green and circular economy, and fighting climate change. It also emphasises the common interests of the EU and the countries of the region, as well as their close collaboration in the international arena for the definition of global agendas, such as the Paris Agreement or the Sustainable Development Goals (SDGs) of the United Nations. © European Union, 2021