Aims:Monitoring rangeland above-ground net primary production (ANPP) requires information on the variation of radiation use efficiency (RUE). We studied the spatial and temporal variations of RUE of the herbaceous layer (RUEH) in woodlands and shrublands and its association with grazing regime, environmental, and vegetation variables. Location:We worked in the Arid Chaco (Argentina), an ecotone region between the Chaco and Monte phytogeographic provinces. Woody and herbaceous species coexist in variable proportions in relation to a regional precipitation gradient and local edaphic variations. Methods and results:Over eight growing seasons, we estimated RUEH at 12 sites, each with two contrasting grazing regimes (moderate and severe). To do this, we related estimated ANPPH from biomass harvests and APARH (absorbed photosynthetically active radiation of the herbaceous layer) calculated from the MODIS normalized difference vegetation index (NDVI). Then we linked the RUEH with precipitation, physiognomy, soil, and landscape data. Most of the spatial variability of RUEH was accounted for by the grazing regime. Moderately grazed areas had a three times greater RUEH than severely grazed ones (0.507 and 0.180 g MJ−1 respectively). They also exhibited different proportions of herbaceous functional types. Physiognomic characteristics of the woody layer explained a significant proportion of the spatial variation of RUEH not explained by grazing regime. RUEH was between 30 and 150% greater in woodlands than in shrublands depending on whether the sites were under moderate or severe grazing respectively. Conclusion:Grazing regime and woody physiognomy accounted for most of the variation in herbaceous radiation use efficiency. Our results show that RUEH may be predicted with greater accuracy from a combination of vegetation maps and information on grazing regime. Thus, our models could be incorporated into web platforms that provide ANPPH (forage) monitoring services based on satellite data, in order to improve their estimates in woodlands, shrublands and savanna ecosystems.