ABSTRACT:In semiarid agricultural systems, irrigation may increase soil organic matter (SOM) due to higher biomass production. However, aboveground production tends to increase more than belowground biomass, and in no-till systems SOM responses to irrigation would strongly depend on the fate of crop residues left on the soil surface. Litter decomposition and SOM formation can also be affected by irrigation due to changes in water dynamics, but little is known about the impacts of irrigation on the pathways of SOM formation. We used dual 13C and 15N labeled maize litter to quantify the effect of irrigation on the contribution of aboveground crop residues to SOM formation in semiarid, no-till agricultural systems. We incubated the litter in a continuous maize cropping system with two treatments, dryland and irrigated, and measured litter decomposition and its contribution to different SOM pools. Irrigation had a larger effect on litter C and N losses than on the formation of new SOM. After 13 months the C and N losses from the litter layer were 24% higher under irrigation, but there were no treatment differences in the litter-derived C and N recovered in SOM. Most of the litter-derived organic matter (OM) was found in the mineral associated OM pool (MAOM), but irrigation did not affect the amount of litter-derived OM found as MAOM or as particulate organic matter. Irrigation increased the amount of litter-derived OM found in macroaggregates, and this observation was more related to a higher aggregation than to aggregate enrichment in the irrigated treatment. Our results suggest that a smaller proportion of aboveground crop residues will form SOM in irrigated systems compared to dryland conditions and may help to partially explain why irrigation has a stronger relative effect on crop productivity and C inputs than on SOC stocks in semiarid agricultural systems.