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A strategy to recover a high-quality, complete plastid sequence from low-coverage whole-genome sequencing.

Enviado por Anónimo (no verificado) el
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ABSTRACT. Premise of the study:We developed a bioinformatic strategy to recover and assemble a chloroplast genome using data derived from low-coverage 454 GS FLX/Roche whole-genome sequencing. Methods:A comparative genomics approach was applied to obtain the complete chloroplast genome from a weedy biotype of rice from Uruguay. We also applied appropriate filters to discriminate reads representing novel DNA transfer events between the chloroplast and nuclear genomes. Results:From a set of 295,159 reads (96 Mb data), we assembled the chloroplast genome into two contigs.

Hot topic: Use of genomic recursions in single-step genomic best linear unbiased predictor (BLUP) with a large number of genotypes.

Enviado por Anónimo (no verificado) el
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ABSTRACT. The purpose of this study was to evaluate the accuracy of genomic selection in single-step genomic BLUP (ssGBLUP) when the inverse of the genomic relationship matrix (G) is derived by the "algorithm for proven and young animals" (APY). This algorithm implements genomic recursions on a subset of "proven" animals. Only a relationship matrix for animals treated as "proven" needs to be inverted, and the extra costs of adding animals treated as "young" are linear. Analyses involved 10,102,702 final scores on 6,930,618 Holstein cows.

Hot topic:Use of genomic recursions in single-step genomic best linear unbiased predictor (BLUP) with a large number of genotypes.

Enviado por Anónimo (no verificado) el
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ABSTRACT. The purpose of this study was to evaluate the accuracy of genomic selection in single-step genomic BLUP (ssGBLUP) when the inverse of the genomic relationship matrix (G) is derived by the "algorithm for proven and young animals" (APY). This algorithm implements genomic recursions on a subset of "proven" animals. Only a relationship matrix for animals treated as "proven" needs to be inverted, and the extra costs of adding animals treated as "young" are linear. Analyses involved 10,102,702 final scores on 6,930,618 Holstein cows.

Taller Sobre Intensificación Sostenible en Ganadería Familiar.

Enviado por Anónimo (no verificado) el
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PRÓLOGO. En el marco de la ejecución del Proyecto Mejora en la Sostenibilidad de la Ganadería Familiar de Uruguay (UFFIP), AgResearch (Nueva Zelanda), el Instituto Plan Agropecuario (IPA), el Instituto Nacional de Investigación Agropecuaria (INIA) y el Ministerio de Ganadería, Agricultura y Pesca (MGAP) organizaron el Taller denominado «Intensificación sostenible de la ganadería familiar:oportunidades y limitantes». El Taller se planteó como objetivo:abordar la discusión de las oportunidades y limitantes de la intensificación sustentable en los sistemas de producción ganaderos familiares,

Use of genomic recursions and algorithm for proven and young animals for single-step genomic BLUP analyses - a simulation study.

Enviado por Anónimo (no verificado) el
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ABSTRACT. The purpose of this study was to examine accuracy of genomic selection via single-step genomic BLUP (ssGBLUP) when the direct inverse of the genomic relationship matrix (G) is replaced by an approximation of G1 based on recursions for young genotyped animals conditioned on a subset of proven animals, termed algorithm for proven and young animals (APY). With the efficient implementation, this algorithm has a cubic cost with proven animals and linear with young animals. Ten duplicate data sets mimicking a dairy cattle population were simulated.

Accuracy of estimated breeding values with genomic information on males, females, or both: An example on broiler chicken.

Enviado por Anónimo (no verificado) el
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ABSTRACT. Background: As more and more genotypes become available, accuracy of genomic evaluations can potentially increase. However, the impact of genotype data on accuracy depends on the structure of the genotyped cohort. For populations such as dairy cattle, the greatest benefit has come from genotyping sires with high accuracy, whereas the benefit due to adding genotypes from cows was smaller. In broiler chicken breeding programs, males have less progeny than dairy bulls, females have more progeny than dairy cows, and most production traits are recorded for both sexes.

Accuracy of estimated breeding values with genomic information on males, females, or both:An example on broiler chicken.

Enviado por Anónimo (no verificado) el
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ABSTRACT. Background:As more and more genotypes become available, accuracy of genomic evaluations can potentially increase. However, the impact of genotype data on accuracy depends on the structure of the genotyped cohort. For populations such as dairy cattle, the greatest benefit has come from genotyping sires with high accuracy, whereas the benefit due to adding genotypes from cows was smaller. In broiler chicken breeding programs, males have less progeny than dairy bulls, females have more progeny than dairy cows, and most production traits are recorded for both sexes.

Phylogenetic studies of the three RNA silencing suppressor genes of south american ctv isolates reveal the circulation of a novel genetic lineage.

Enviado por Anónimo (no verificado) el
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ABSTRACT. Citrus Tristeza Virus (CTV) is the most economically important virus of citrus worldwide. Genetic diversity and population structure of CTV isolates from all citrus growing areas from Uruguay were analyzed by RT-PCR and cloning of the three RNA silencing suppressor genes (p25, p20 and p23). Bayesian phylogenetic analysis revealed the circulation of three known genotypes (VT, T3, T36) in the country, and the presence of a new genetic lineage composed by isolates from around the world, mainly from South America.