Abstract: Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ?Génesis 2375?. The most resistant lines to both diseases are expected to be valuable genetic resources in breeding for durable resistance to FHB and SR.