ABSTRACT. Techniques and methods based on vibrational spectroscopy such as near-infrared refl ectance (NIR), mid-infrared (MIR) and Raman spectroscopy are known to be non-destructive and low cost.These characteristics are considered as the most important when these methods or techniques are applied in the fi eld of plant omics. This chapter will provide an overview of the most common vibrational spectroscopy techniques used in the fi eld of plant omic analysis (NIR, MIR, Raman). Information about the hardware (instruments) and software (multivariate data methods) will be also presented and discussed. © Springer India 2015.