ABSTRACT.- In the summer of 2023, ingestion of Astylus atromaculatus (pollen beetle) was linked to spontaneous fatal disease in grazing cattle and sheep in Argentina and Uruguay. While the disease was experimentally reproduced in sheep and guinea pigs in the 1970's, no experimental reproductions have been attempted in cattle, and controversy exists as to whether this insect is indeed noxious to cattle and at which dose. Here, we demonstrate that A. atromaculatus causes acute fatal disease in Hereford calves at single oral dosages of 2.5, 4.5, 10.0, and 15.0 g of insect/kg body weight.

ABSTRACT.- Under the EU Renewable Energy Directive (2009/28/EC), estimating biofuel GHG emissions is essential to gauge emissions reductions compared to fossil fuels. Within this framework, the carbon footprint (CF) was calculated for four bioethanol cropping systems:a maize-wheat-sorghum rotation without the harvest of crop residues (MWS), a maize-wheat-sorghum rotation with harvested crop residues (MWS-R), switchgrass (Sw), and continuous sweet sorghum (Ss). The estimation followed a life-cycle analysis strategy. The CF varied between 0.04 and 3.68 kg CO2-eq l -1 ethanol.