Pseudomonas spp. isolates were collected from tomato plants showing symptoms of pith necrosis during the 2017 and 2018 seasons in the Salto Department, northwest Uruguay. According to physiological, biochemical tests and multilocus phylogenetic analysis (gyrB, rpoD, and rpoB), 25 were characterized as P. mediterranea, five as P. viridiflava, three as P. corrugata, and one as P. marginalis. All of them produced necrotic lesions and pith hollowing in re-inoculated tomato plants. Some P.